Medical Cell Biology | Molecular Cell Biology | Cell Maintenance | Stem Cell Biology |
Cell Differentiation | Cell Modulation | Pattern Formation | Brain Morphogenesis |
Kidney Development | Organ Formation |
Equipment | Manufacture, product ID | Location room number | Application | Contact person |
Electroporator | NEPA gene CUY21, CUY21SC | 608 | Gene transfer device. It transfers genes with energizing into chicken embryos and mouse embryos |
Jun Hatakeyama (6585) |
Vibrating blade microtome | LEICA(VT1200S) DOSAKA(DTK-1000) |
608 | It slices tens of ƒÊm to hundreds of ƒÊm thickness. | Jun Hatakeyama (6585) |
Timelapse observing system (multi point) |
OLYMPUS(invert ed microscope), Molecular device etc. |
607 | It can observe timeplase of sample fluorescence signals on 35mm petri dish. | Jun Hatakeyama (6585) |
Micropippet puller | Sutter Instrument | 608 | It produces needles from glass capillary. | Jun Hatakeyama (6585) |
Method | Description | Contact person |
Electroporation (chick embryo) |
It is the method to introduce genes energizing to chick embryo in vivo. | Hatakeyama (6585) |
Electroporation (mouse embryo) |
It is the method to introduce genes energizing to mouse embryo in vivo. | Hatakeyama (6585) |
Micro electroporation | It is the method to introduce genes into spots of 10 to 20 cells. | Hatakeyama (6585) |
Whole embryo culture (chick embryo, mouse embryo) |
It is the technique to culture whole embryo (chick and mouse). It allows embryo operation which difficult in ovo or in utero. |
Shimamura/ Hatakeyama (6585) |
Organ culture (chick and mouse embryonic tissues) |
It is the technique to culture the neural tissues isolated from chick embryo and mouse embryo. | Shimamura/ Hatakeyama (6585) |
Timelapse imaging | It is the technique to analyze cellular behavior in the slice culture of the neural tissues of mouse embryo by a timelapse microscope. | Hatakeyama (6585) |
Chick experimental embryology (microsurgery) | Microsurgery for transplantations. | Hatakeyama (6585) |
Electron microscope (SEM,TEM) |
Observation for zebrafish, chick and mouse embryos with a scanning electron microscope (SEM) and a transmission electron microscopes (TEM). | Hatakeyama (6585) |
Immuno electron microscopy (SEM,TEM) |
Observation for immunostained chick and mouse embryos with a scanning electron microscope and a transmission electron microscopes. | Hatakeyama (6585) |