Medical Cell Biology | Molecular Cell Biology | Cell Maintenance | Stem Cell Biology |
Cell Differentiation | Cell Modulation | Pattern Formation | Brain Morphogenesis |
Kidney Development | Organ Formation |
Equipment | Manufacture, product ID | Location room number | Application | Contact person |
Ventana Discovery | Roche, Disvovery | 507 | Automated slide-based application for in situ hybridization and immunohistochemistry | Tomoko Ohmori (6616) |
Automated micro-injector | eppendorf, FemtoJet | 405 | Semiautomatic injection into cells with a small volume | Chiyoko Kobayashi (6617) |
Manual micro-injector | NARISHIGE, NM-202 | 405 | Manual micro-injector(for injecting DNA into the mouse embryo) | Satomi Tanaka (6617) |
electroporator | Bio-RAD, gene pulser X cell | 405 | For gene transfer into ES cells | Ryuichi Nishinakamura (6615) |
electroporator | BEX, CUY21 | 405 | Square pulse generator (for in vivo electroporation) | Satomi Tanaka (6617) |
luminometer | Lumat LB9507 | 407 | Tube type luminescence meter (for luciferase assay) | Satomi Tanaka (6617) |
LAS | FUJI FILM, LAS-3000 | 406 | Luminescence imaging analyzer (for detecting the western blot signal) | Masaji Sakaguchi (6617) |
Vacuum rotary | Sakura Finetek, VRX-23A | 406 | Automatic vacuum rotary for dehydration and the tissue embedding into paraffin | Tomoko Ohmori (6616) |
Time-lapse system | OLYMPUS IX70 | 406 | Time-lapse image capturing system with inverted microscope | Ryuichi Nishinakamura (6615) |
Method | Description | Contact person |
Elelctroporation of DNA into the cultured mouse embryo | Elelctroporation and whole mouse embryo culture | Satomi Tanaka (6617) |
Culture of ES cells | Gene targeting by the homologous recombination in E14.1 cells | Ryuichi Nishinakamura (6615) |
in situ hybridization | Section in situ hybridization (paraffin section) using Ventana Discovery | Ryuichi Nishinakamura (6615) |
Southern blot | Non-RI Genomic Southern blot | Ryuichi Nishinakamura (6615) |